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KMID : 0378019820250040079
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New Medical Journal
1982 Volume.25 No. 4 p.79 ~ p.89
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Studies on the Acid-Fast Bacilli Isolated from Natural Source
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金聖光/KIM, Sung-Kwang
金世鍾/鄭聖德/金周德/KIM, Se-Jong/CHEUNG, Sung-Douk/KIM, Joo-Deuk
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Abstract
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Each one strain of 10 species of atypical mycobacteria isolated from soils at Seoul and Hiroshima was inoculated into dd-Y mice to test and compare the pathogenicity with that of Mycobacterium tuberculosis. The species tested were M. scrofulaceum, M. gordonae, M. agri, M. smegmatis, M. aurum, M. fortuitum, M. terrae, M. chelonei sub chelonei, M. thermoresistibile and M. nonchrornogenicum.
Each strain was harvested from young bacterial mass on 1% Ogawa medium and suspe￢nded in sterile distilled water containing 0. 1% Tween 80 at the concentration of 1 mg per milliliter. A 0.2 ml of the suspension was inoculated into mice intravenously.
The sequential changes in body weight of mice were recorded at appropriate intervals after inoculation. Two mice from each animal group were sacrificed every week to examine the gross and histopathological changes in internal organs. A 50mg of tissues from each organ was cultured to recover the infected acid-fast bacilli. These observation were continued until 6 weeks after inoculation. Susceptibility of the bacilli thus recovered to antituberculosis drugs were tested in vitro.
The results obtained are as follows:
1) Increase-in body weight of mice infected ;with atypical mycobacteria was slower ´than that in the non-infected controls. No significant difference, however was found among these groups. The mice infected with M. tuberculosis gained weight much less than the others.
:2) At autopsies, the macroscopic;-lesions occurred on the organs of the mice infected with M. tuberculosis and advanced progressively. The mice infection with M. scrofulaceum, M. smegmatis, M. fortuitum, M.chelonei sub chelonei, M.thermoresistibile or M.nonchromogenicum developed slight changes which were only temporary and .´diminished spontaneously. No gross pathologic changes were noticed in the animals infected with M gordonae, M. aurum or M. terrae.
3) Root indices of the weight of spleen and ´lungs were calculated at more than 1. 0 and 10. 0 value with the animals infected with M. tuberculosis 3 weeks after infection. Among the atypical mycobacteria groups, the values were significant temporailly with the mice 2 and 3 weeks after infection with M. scrofulaceum or M. fortuium.
4) Acid-fast bacilli were always successfully recovered from the all organs of the mice in M. tuberculosis group from 2 to 6 weeks after infection. Recovery was also positive in some cases among the atypical mycobacteria groups except the mice in the groups of M. gordonae, M. aurum and M. terrae, but all turned negative 6 weeks after infection.
5) All strains recovered were uniformly resistant to PAS, SM and INH, and, in general, also resistant to TH, CS, EB and RFP. However, some strains were susceptible to those 4 antibiotics in certain degree, and, furthermore, some were inhibited severely by EB or RFP.
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KEYWORD
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